NDLI: Anticarsia gemmatalis ( Hübner 1818 ) ( Lepidoptera : Erebidae ) Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis ( Hübner 1818 ) ( Lepidoptera : Erebidae ) larvae

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Anticarsia gemmatalis ( Hübner 1818 ) ( Lepidoptera : Erebidae ) Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis ( Hübner 1818 ) ( Lepidoptera : Erebidae ) larvae

Content Provider	Semantic Scholar
Author	Cruz, Norberto Aparecido Hoffmann-Campo, Clara Beatriz Levy, Sheila Michele Ângela Falleiros, Maria Ferreira
Copyright Year	2018
Abstract	1 Doutorado em Agronomia pela Universidade Estadual de Londrina. Centro de Ciências Agrárias, Universidade Estadual de Londrina; Londrina, Paraná, Brasil. 2 Doutorado em Botânica pela The University Of Reading, Grã-Bretanha. Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Soja; Londrina, Paraná, Brasil. 3 Doutorado em Ciências Biológicas (Zoologia) pela Universidade Estadual Paulista Júlio de Mesquita Filho. Centro de Ciências Biológicas, Departamento de Histologia, Universidade Estadual de Londrina; Londrina, Paraná, Brasil. 4 Doutorado em Ciências Biológicas (Anatomia Humana) pela Universidade Estadual Paulista Júlio de Mesquita Filho. Centro de Ciências Biológicas, Departamento de Histologia, Universidade Estadual de Londrina; Londrina, Paraná, Brasil. E-mail: angefal@gmail.com DOI: 10.5433/1679-0367.2017v38n2p185 Um controle alternativo para a lagarta da soja tem sido o uso de plantas resistentes a insetos que contém flavonoides fenólicos. O intestino médio é a principal via de acesso do alimento e substâncias químicas ingeridas. O presente estudo examinou as alterações morfológicas no epitélio do intestino médio da lagarta da soja, após a ingestão de genótipos de soja contendo os flavonoides rutina e genistina. As lagartas e os genótipos (BRS 257 controle, BR 16, Dowling, PI 229358, IAC 100 e PI 227687) foram obtidos do laboratório de criação de insetos da Embrapa Soja. Os intestinos médios foram coletados, fixados em Karnovsky, processados e analisados ao microscópio de luz. Todos os tratamentos causaram alterações nas células epiteliais do intestino médio. As alterações foram mais assíduas nas células colunares do que nos demais tipos celulares. Essas apresentaram grande quantidade de protrusões citoplasmáticas e de vacúolos. As células caliciformes apresentaram poucas alterações para todos os genótipos testados, enquanto as células regenerativas apresentaram alterações principalmente nos tratamentos Dowling e PI 227687. A membrana peritrófica estava ausente para os genótipos IAC100 e PI 227687. Conclui-se que os genótipos Dowling e PI 227687 foram bastante efetivos e promoveram grandes alterações morfológicas no intestino médio das lagartas da soja, podendo ser bastante eficaz para o controle desta praga. ARTIGOS / ARTICLES C ÊN C AS IO LÓ G IC AS E D A AÚ D E Palavras chave: Lagarta da soja. Morfologia. Tubo digestivo. Rutina e genistina. An alternative control to soybean caterpillar has been the use of insect resistant plants that present phenolic flavonoids. The midgut is the main access pathway of food and chemical substances ingested. The present study examined morphological alterations in the midgut epithelium of the soybean caterpillar, after the ingestion of soybean genotypes containing the flavonoids rutin and genistin. The caterpillars and genotypes (BRS 257 control, BR 16, Dowling, PI 229358, IAC 100 and PI 227687) were obtained from the insect rearing facility at the Embrapa Soja. The midguts were collected, fixed in Karnovsky, processed and analyzed under a light microscope. All treatments caused alterations in the midgut epithelial cells. These alterations in the columnar cells were more assiduous than the on other cell types, showing an increase of cytoplasmic protrusions and vacuoles. The goblet cells showed few changes for all genotypes tested, while the regenerative cells presented alterations mainly for the Dowling and PI 227687 treatments. The peritrophic membrane was absent for genotypes IAC100 and PI 227687. We conclude that the Dowling and PI 227687 genotypes were effective and promoted great morphological alterations in the midgut of the soybean caterpillars, being able to be very effective for the control of this plague. Resumo Keyword: Soybean caterpillar. Morphology. Digestive tract. Rutin and genistin.
File Format	PDF HTM / HTML
DOI	10.5433/1679-0367.2017v38n2p185
Alternate Webpage(s)	http://www.uel.br/revistas/uel/index.php/seminabio/article/download/30119/23168
Alternate Webpage(s)	https://doi.org/10.5433/1679-0367.2017v38n2p185
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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