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Molecular basis of cooperative DNA bending and oriented heterodimer binding in the NFAT1-Fos-Jun-ARRE2 complex.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Diebold, Ronald J. Rajaram, Nirmala Leonard, David A. Kerppola, Tom Klaus |
| Copyright Year | 1998 |
| Abstract | Cooperative DNA binding by transcription factors that bind to separate recognition sites is likely to require bending of intervening sequences and the appropriate orientation of transcription factor binding. We investigated DNA bending in complexes formed by the basic region-leucine zipper domains of Fos and Jun with the DNA binding region of nuclear factor of activated T cells 1 (NFAT1) at composite regulatory elements using gel electrophoretic phasing analysis. The NFAT1-Fos-Jun complex induced a bend at the ARRE2 site that was distinct from the sum of the bends induced by NFAT1 and Fos-Jun separately. We designate this difference DNA bending cooperativity. The bending cooperativity was directed toward the interaction interface between Fos-Jun and NFAT1. We also examined the influence of NFAT1 on the orientation of Fos-Jun heterodimer binding using a novel fluorescence resonance energy transfer assay. The interaction with NFAT1 could reverse the orientation of Fos-Jun heterodimer binding to the ARRE2 site. The principal determinants of both cooperative DNA bending and oriented heterodimer binding were localized to three amino acid residues at the amino-terminal ends of the leucine zippers of Fos and Jun. Consequently, interactions between transcription factors can remodel promoters by altering DNA bending and the orientation of heterodimer binding. |
| Starting Page | 578 |
| Ending Page | 578 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/95/14/7915.full.pdf |
| PubMed reference number | 9653115v1 |
| Volume Number | 95 |
| Issue Number | 14 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Bending - Changing basic body position Decompression Sickness Energy Transfer Fluorescence Introns JNK Mitogen-Activated Protein Kinases Leucine Zippers TRANSCRIPTION FACTOR dna binding promoter |
| Content Type | Text |
| Resource Type | Article |
