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Strain specific variation in IFN-γ inducible lymphocyte adhesion to rat brain endothelial cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Linke, A. T. Greenwood, John Campbell, I. Luthert, Pj Male, David Kingsley |
| Copyright Year | 1998 |
| Abstract | Abstract We have examined the interferon-gamma (IFN-γ) induced increase in lymphocyte adhesion to rat brain endothelial cells (BEC) in the experimental allergic encephalomyelitis (EAE) susceptible LEW and resistant PVG strain. A significant increase in adhesion of mitogen activated lymphocytes could be demonstrated by stimulating LEW BEC with 50 U/ml IFN-γ for 24 h. In contrast the same treatment failed to induce a significant increase in lymphocyte adhesion in the PVG strain. Flow cytometric analysis of lymphocyte integrin expression indicated a marked increase in the number of cells expressing both LFA-1 and VLA-4 following mitogen activation and was similar between the LEW and PVG strain. Depletion of LFA-1 and VLA-4 positive lymphocytes resulted in an equivalent inhibition of adhesion to BBB-EnC indicating that the adherent population was comprised predominantly of the VLA-4+/LFA-1+ phenotype. We conclude that this strain variation in the IFN-γ activation of BEC may be related to the disease phenotypes of these strains. |
| Starting Page | 28 |
| Ending Page | 32 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0165-5728(98)00152-0 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0165572898001520 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0165572898001520?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0165-5728%2898%2900152-0 |
| Volume Number | 91 |
| Journal | Journal of Neuroimmunology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
