Lipopolysaccharide-induced change of ADP-ribosylation of a cytosolic protein in bone-marrow-derived macrophages.

Content Provider	Semantic Scholar
Author	Hauschildt, Sunna Scheipers, Peter Bessler, Wolfgang
Copyright Year	1994
Abstract	Treatment of bone-marrow-derived macrophages with nanogram quantities of bacterial lipopolysaccharide (LPS) or with the synthetic bacterial lipopeptide analogue N-palmitoyl-(S)-[2,3-bis(palmitoyloxy)-(2RS)-propyl] (Pam3)Cys-Ala-Gly results in a change of ADP-ribosylation of a cytosolic 33 kDa protein. The immunostimulant-induced change is both dose- and time-dependent. It is not observed in macrophages from an LPS-unresponsive C3H/HeJ mouse strain upon treatment with LPS. Non-endotoxic LPS from Rhodopseudomonas pallustris, the inactive lipopeptide analogue Pam3CysOH, and LPS in the presence of polymyxin B fail to induce the change of ADP-ribosylation of the protein. These observations indicate that reversible protein modification by ADP-ribosylation might play a role in macrophage activation.
Starting Page	17
Ending Page	20
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.biochemj.org/content/ppbiochemj/297/1/17.full.pdf
PubMed reference number	8280095v1
Volume Number	297
Part	1
Journal	The Biochemical journal
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Diphosphate Analog Bone Tissue Immunologic Adjuvants Lipopolysaccharides Macrophage Activation Mice, Inbred C3H Polymyxin B Polymyxins Post-Translational Protein Processing Quantity nanogram serine-type D-Ala-D-Ala carboxypeptidase
Content Type	Text
Resource Type	Article