NDLI: Validation of the in vivo Iodo-Nitro-Tetrazolium (INT) Salt Reduction Method as a Proxy for Plankton Respiration

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Validation of the in vivo Iodo-Nitro-Tetrazolium (INT) Salt Reduction Method as a Proxy for Plankton Respiration

Content Provider	Semantic Scholar
Author	García-Martín, E. Elena Aranguren-Gassis, María Karl, David Martínez-García, Sandra Robinson, Carol Serret, Pablo Teira, Eva
Copyright Year	2019
Abstract	Knowledge of the magnitude and variability of plankton respiration is a crucial gap in our understanding of marine carbon cycling. In order to validate the INT reduction method as a proxy for plankton respiration, we have compiled and analysed a dataset (n = 376) of concurrent measurements of dissolved oxygen consumption (CRO2) and in vivo reduction of 2-para (iodophenyl)-3(nitrophenyl)-5(phenyl) tetrazolium chloride tetrazolium salt (INT) spanning a wide range of oceanic regions and physicochemical conditions. Data were randomly divided into two independent subgroups: two thirds of the data were used to derive a regression conversion between dissolved oxygen consumption and INT reduction (“training” dataset) and one third of the data was used to validate the regression (“test” dataset). There was a significant relationship between the log-transformed dissolved oxygen consumption rates and the log-transformed INT reduction rates (INTT) with the “training” dataset (logCRO2 = 0.72logINTT + 0.44, R2 = 0.69, n = 249, p 8 ˚C and chlorophyll-a concentrations >0.2 µg L-1 and reduced at lower temperatures and chlorophyll-a concentrations. The results of this study endorse the adequate performance and reliability of the INT method for natural plankton communities.
File Format	PDF HTM / HTML
DOI	10.3389/fmars.2019.00220
Volume Number	6
Alternate Webpage(s)	https://ueaeprints.uea.ac.uk/70920/1/Published_Version.pdf
Alternate Webpage(s)	https://doi.org/10.3389/fmars.2019.00220
Journal	Front. Mar. Sci.
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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