NDLI: Conformation of double-stranded polydeoxynucleotides in solution by proton two-dimensional nuclear Overhauser enhancement spectroscopy.

Content Provider	Semantic Scholar
Author	Borah, Babul Cohen, Jack S. Bax, Adriaan
Copyright Year	1985
Abstract	Proton 2D-NOE spectroscopy has been used to investigate the three-dimensional conformations of several sonicated polydeoxynucleotides in solution. The observed pattern of cross peaks indicate that poly(dA-dT) · poly(dA-dT) in all salt concentrations studied (up to 6.6M CsF), and poly(dG-m5dC) · poly(dG-m5dC) in low salt (0.1M NaCl) are righthanded B-structures. Poly(dG-m5dC) · poly(dG-m5dC) in Mg2+ (3 mM) solution exhibits a pattern characteristic of the left-handed Z-form. These results for poly(dA-dT) · poly(dA-dT) are in contrast to suggestions that this copolymer exists as a left-handed form, either in low or high salt. We present pure absorption-mode 2D-NOE spectra that enable us to compare several distances and define the conformations of these polydeoxynucleotides in solution.
File Format	PDF HTM / HTML
DOI	10.1002/bip.360240503
PubMed reference number	4016214
Journal	Medline
Volume Number	24
Issue Number	5
Alternate Webpage(s)	https://spin.niddk.nih.gov/bax/lit/508/47.pdf
Alternate Webpage(s)	https://doi.org/10.1002/bip.360240503
Journal	Biopolymers
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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