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Shoot Multiplication and Plant Regeneration from In Vitro Cultures of Drumstick Tree ( Moringa oleifera Lam . )
| Content Provider | Semantic Scholar |
|---|---|
| Author | Riyathong, Thidarat Srisulak Dheeranupattana Palee, Jiraporn Shank, Lalida |
| Copyright Year | 2012 |
| Abstract | To obtain plantlets of drumstick tree (Moringa oleifera Lam.) with identical genetic make up for propagation or field studies, in vitro cultures of drumstick tree were produced. For multiple shoot induction, the stem explants of drumstick tree plantlet were cultured on Murashige and Skoog (MS) agar medium supplemented with different concentrations of 6-benzyl adenine purine (BA) and maintained at 25 ± 2°C for 5 weeks. Multiple shoot induction was present in the stem explants that were cultured on the MS medium supplemented with BA at the concentration range of 1.0 4.0 mg/l. The MS medium containing BA at 2.0 mg/l was found to produce 100% shoot formation with the highest average number of 10.8 shoots per explant. For plant regeneration, the shoot explants were cultured on MS agar medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.5 mg/l for callus production. Friable callus were transferred to MS agar medium supplemented with different concentrations of 1-napthalene acetic acid (NAA) or BA for 4 weeks. Callus grown in MS medium supplemented with BA (0 – 2.0 mg/l) did not produce either shoots or roots, but showed green spots on the surface of callus. Callus grown in MS medium containing NAA 0.5 mg/l produced both shoots and roots. While, only root production were found in the medium supplemented with NAA 1.0 mg/l. Moreover, when NAA concentration was raised to 2.0 mg/l green spots appeared on the surface of callus without further developing into the whole plants. The production of multiple shoots provided materials for callus production study which led to regeneration of plantlets with both roots and shoots. Tissue culture protocol reported in this study is an alternative mean of propagation of drumstick plantlets with uniform genotypes for breeding selection and field experiments. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.sci.kmitl.ac.th/Downloads/proceeding_2/BCBT2010%20%20%20PDF/26%20page154-159.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
