Loading...
Please wait, while we are loading the content...
Similar Documents
A disintegrin and metalloprotease-12 is type I myofiber specific in Bos taurus and Bos indicus cattle.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Coles, Chantal A. Wadeson, Jenny Knight, Matthew I. Cafe, Linda Maree. Johns, William H. White, Jennifer Daniels Greenwood, Paul L. McDonagh, Matthew B. |
| Copyright Year | 2014 |
| Abstract | A disintegrin and metalloproteinase-12 (ADAM12) is involved in the regulation of myogenesis and adipogenesis and is of interest as a potential target to manipulate skeletal muscle development and intramuscular fat (IMF) deposition in cattle to increase beef yield and improve meat quality. The longissimus thoracis muscle (LM) and semitendinosus muscle (STM) from 5 Bos taurus (Angus) and 5 Bos indicus (Brahman) cattle were collected for histological and ADAM12 gene and protein expression analysis. Myofiber typing was used to determine if ADAM12 expression patterns related to differences in muscling and IMF deposition, which are influenced by proportions of the different myofiber types. The STM was found to contain a higher proportion of glycolytic myofibers than the LM, which contained a greater proportion of oxidative myofibers (myofiber ratio of glycolytic to more oxidative types in LM and STM of 1.1 and 3.5, respectively; P < 0.05). ADAM12 gene expression, fluorescent immunohistochemical staining for ADAM12, and image analysis found ADAM12 to be greater in the LM (P < 0.05). Regression analysis found a strong, positive relationship for the distribution of ADAM12 against the proportion of type I myofibers (P < 0.05, r(2) = 0.86). These findings suggest ADAM12 is upregulated in muscles with more slow-oxidative myofibres, such as the LM, and is linked to type I myofibers in cattle. ADAM12 may be important in the regulation and maintenance slow myofibers in the LM muscle. |
| Starting Page | 1023 |
| Ending Page | 1028 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.animalsciencepublications.org/publications/jas/pdfs/92/4/1473 |
| PubMed reference number | 24663211v1 |
| Alternate Webpage(s) | https://doi.org/10.2527/jas.2013-7069 |
| DOI | 10.2527/jas.2013-7069 |
| Journal | Journal of animal science |
| Volume Number | 92 |
| Issue Number | 4 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ADAM12 gene Adipogenesis Bos taurus Contain (action) Fatty acid glycerol esters Gene Expression Muscle Development Myogenesis Platelet Glycoprotein 4, human Semitendinosus muscle structure Staining method Structure of longissimus thoracis muscle |
| Content Type | Text |
| Resource Type | Article |
