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Printing biomacromolecules on a bovine serum albumin precursor layer.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Bo Feng, Jie Gao, Changyou |
| Copyright Year | 2005 |
| Abstract | Various biomacromolecules including proteins and polysaccharides are printed on a substrate capped with a bovine serum albumin (BSA) precursor layer to create clear co-patterns of these molecules. Characterizations by confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM) demonstrate the successful production and clear boundaries of the co-patterns. Rinsing the BSA-adsorbed substrate and the biomacromolecules-inked stamp before microcontact printing (microCP) is crucial for the creation of clear and stable co-patterns. The patterns are mainly stabilized by electrostatic interactions and van der Waals forces. Characterizations by ellipsometry, UV-Vis and fluorescence spectroscopy reveal that printing by a flat PDMS stamp yields a denser layered structure of proteins with a higher amount than that of adsorbed proteins. By printing, however, a lower enzymatic catalytic activity for horseradish peroxidase (HRP) or binding capability for avidin (both normalized to amount) is determined. A conformational transition from alpha-helix to beta-sheet of HRP is observed by ATR-IR. By contrast, a BSA precursor layer can effectively improve the functionality of the printed HRP or avidin and preserve the original conformation of the proteins, although the absolute transferred amount of these proteins is decreased. |
| Starting Page | 281 |
| Ending Page | 286 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://polymer.zju.edu.cn/biomaterials/uploads/Publications/2005/041.pdf |
| PubMed reference number | 16080169v1 |
| Volume Number | 5 |
| Issue Number | 8 |
| Journal | Macromolecular bioscience |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ATR protein, human Avidin Cattle Fluorescence Spectroscopy Horseradish Peroxidase Ink (substance) Microscopy, Atomic Force Microscopy, Confocal Polysaccharides Printing, Three-Dimensional Serum Albumin Serum Albumin, Bovine anatomical layer enzyme activity |
| Content Type | Text |
| Resource Type | Article |
