Loading...
Please wait, while we are loading the content...
Similar Documents
Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes
| Content Provider | Semantic Scholar |
|---|---|
| Author | Boonyasit, Yuwadee Heiskanen, Arto Chailapakul, Orawan Laiwattanapaisal, Wanida |
| Copyright Year | 2015 |
| Abstract | We propose a novel alternative approach to long-term glycaemic monitoring using eggshell membranes (ESMs) as a new immobilising platform for the selective label-free electrochemical sensing of glycated haemoglobin (HbA1c), a vital clinical index of the glycaemic status in diabetic individuals. Due to the unique features of a novel 3-aminophenylboronic acid-modified ESM, selective binding was obtained via cis–diol interactions. This newly developed device provides clinical applicability as an affinity membrane-based biosensor for the identification of HbA1c over a clinically relevant range (2.3 - 14 %) with a detection limit of 0.19 %. The proposed membrane-based biosensor also exhibited good reproducibility. When analysing normal and abnormal HbA1c levels, the within-run coefficients of variation were 1.68 and 1.83 %, respectively. The run-to-run coefficients of variation were 1.97 and 2.02 %, respectively. These results demonstrated that this method achieved the precise and selective measurement of HbA1c. Compared with a commercial HbA1c kit, the results demonstrated excellent agreement between the techniques (n = 15), demonstrating the clinical applicability of this sensor for monitoring glycaemic control. Thus, this low-cost sensing platform using the proposed membrane-based biosensor is ideal for point-of-care diagnostics. |
| Starting Page | 5287 |
| Ending Page | 5297 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s00216-015-8680-8 |
| Alternate Webpage(s) | http://orbit.dtu.dk/en/publications/selective-labelfree-electrochemical-impedance-measurement-of-glycated-haemoglobin-on-3aminophenylboronic-acidmodified-eggshell-membranes(d2e3f197-e21e-43d8-bed8-6187982f89cf).pdf?nofollow=true&rendering=standard |
| Alternate Webpage(s) | http://orbit.dtu.dk/en/publications/selective-labelfree-electrochemical-impedance-measurement-of-glycated-haemoglobin-on-3aminophenylboronic-acidmodified-eggshell-membranes(d2e3f197-e21e-43d8-bed8-6187982f89cf).pdf?nofollow=true&rendering=mla |
| PubMed reference number | 25956596 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00216-015-8680-8 |
| Journal | Medline |
| Volume Number | 407 |
| Journal | Analytical and Bioanalytical Chemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
