NDLI: MDR1 Up-Regulated by Apoptotic Stimuli Suppresses Apoptotic Signaling

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MDR1 Up-Regulated by Apoptotic Stimuli Suppresses Apoptotic Signaling

Content Provider	Semantic Scholar
Author	Sakaeda, Toshiyuki Nakamura, Tsutomu Hirai, Midori Kimura, Takashi Wada, Atsushi Yagami, Tatsurou Kobayashi, Hironao Nagata, Shunji Okamura, Noboru Yoshikawa, Takayoshi Shirakawa, Toshiro Gotoh, Akinobu Matsuo, Masafumi Okumura, Katsuhiko
Copyright Year	2004
Abstract	AbstractPurpose. Recently, MDR1 (P-glycoprotein) and related transporters have been suggested to play a fundamental role in regulating apo- ptosis, but little information is available concerning the role of MDR1. Here, the effect of apoptotic stimuli on the MDR1 mRNA and apoptotic signaling was examined in MDR1-overexpressing cells. Methods. The expression levels of mRNA for MDR1, MRP1, MRP2, p53, p21, Bax, and Bcl-2 were measured by real time quantitative polymerase chain reaction in HeLa and its MDR1-overexpressing sublines. The effects of apoptotic stimuli by cisplatin (CDDP) on their levels were also assessed as well as on caspase 3, 8, and 9 activities. Results. MDR1 was rapidly upregulated when the cells were exposed to apoptotic stimuli by CDDP. The increase in Bax mRNA to Bcl-2 mRNA ratio after treatment with CDDP was suppressed in MDR1-overexpressing cells. The increases in caspase 3 and 9 activities after treatment with CDDP were suppressed in MDR1-overexpression cells. Conclusion. MDR1 is upregulated by apoptotic stimuli suppressed apoptotic signaling presumably via the mitochondrial pathway.
Starting Page	1323
Ending Page	1329
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1023/A:1020302825511
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1023/A:1020302825511
PubMed reference number	12403069
Alternate Webpage(s)	https://doi.org/10.1023/A%3A1020302825511
Journal	Medline
Volume Number	19
Journal	Pharmaceutical Research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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