Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

Content Provider	Semantic Scholar
Author	Ahmad, Naveed Alatar, Abdulrahman A. Faisal, Mohd Khan, Mohammad Ishaq Fatima, Nigar Anis, Mohammad Hegazy, Ahmad K.
Copyright Year	2014
Abstract	The present study exemplifies morphogenic roles played by copper and zinc during micropropagation of Rauvolfia serpentina, an important medicinal shrub. Incorporation of 20 μM CuSO4 or 25 μM ZnSO4 to a Murashige and Skoog (MS) medium with optimized concentrations of auxins and cytokinins induced a maximum number of shoots per explant (40.67 ± 1.76 and 45.47 ± 0.24, respectively). However, higher concentrations of both the micronutrients negatively affected the morphogenic potential. The pigment content of the regenerants increased up to the optimal concentrations of both metals and thereafter decreased, whereas the maximum proline content was at the highest concentrations used. In vitro rooting of healthy shoots was accomplished using 0.5 μM IBA in a half strength liquid MS medium with 8.20 ± 0.37 roots, and root length of 5.50 ± 0.14 cm per microshoot. The plants survived a hardening procedure and were successfully acclimatized to field conditions with 95 % survival.
Starting Page	11
Ending Page	17
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1007/s10535-014-0479-5
Volume Number	59
Alternate Webpage(s)	https://bp.ueb.cas.cz/pdfs/bpl/2015/01/02.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s10535-014-0479-5
Journal	Biologia Plantarum
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article