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Human macrophage tumor necrosis factor (TNF)-alpha production induced by Trypanosoma brucei gambiense and the role of TNF-alpha in parasite control.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Daulouède, Sylvie Bouteille, Bernard Moynet, Daniel J. Baetselier, Patrick De Courtois, Pierrette Lemesre, J. L. Buguet, Alain Cespuglio, Raymond Vincendeau, Philippe |
| Copyright Year | 2001 |
| Abstract | Trypanosoma brucei gambiense, a causative agent of sleeping sickness, induced a dose-dependent production of tumor necrosis factor (TNF)-alpha by human macrophages in vitro. TNF-alpha was also induced in the Mono Mac 6 cell line, which indicates a direct effect of parasite components on macrophages. Parasite-soluble factors were also potent inducers of TNF-alpha. The addition of anti-TNF-alpha to cocultures of macrophages and parasites increased the number of trypanosomes and their life span, whereas irrelevant antibodies had no effect. TNF-alpha may have a direct role (i.e., direct trypanolytic activity) and/or an indirect one, such as TNF-alpha-mediated induction of cytotoxic molecules. A direct dose-dependent lytic effect of TNF-alpha on purified parasites was observed. This lytic effect was inhibited by anti-TNF-alpha. These data suggest that, as in experimental trypanosomiasis, TNF-alpha is involved in parasite growth control in human African trypanosomiasis. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 11237819 |
| Journal | Medline |
| Volume Number | 183 |
| Issue Number | 6 |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jid/183/6/10.1086/319257/2/183-6-988.pdf?Expires=1492468469&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=JgmCuORsOZ1Irzfu6QJxOeE2~MExbnvrqHRaObYZb~0qLmA68Ryj2An1H8LincvyO4S9TxylSJS6k1K4tnKGW2QRmvTD8A9q3fmz-vhOUgCPemPshfuRtAZ0oyIZHji8avQuf8SXSawbZPmKfr~8B6ushQikDhCD~ZYYQT-RsL1EeDCa0GqzDj9VlVNAvFEipJJ7Kf~24QLzgSFfE6FvYyjAHmp~U-yXQfFS34ccwW7ckNDvRqFMbX1G2TUzHk~U~IRU5XVy5NndN76DwD9iz~0Bxk4npEvuxjRrIHhq3Iw9~zFWm5Q67WkMmLDXW88EpSef0kkL06SKN4H36DsSBw__ |
| Alternate Webpage(s) | http://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_57-58/010025680.pdf |
| Journal | The Journal of infectious diseases |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |