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L-FABP and I-FABP expression increase NBD-stearate uptake and cytoplasmic diffusion in L cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Murphy, Eric J. |
| Copyright Year | 1998 |
| Abstract | The effects of intestinal and liver fatty acid binding protein (I- and L-FABP, respectively) expression on single-cell fatty acid uptake, internalization, and cytoplasmic diffusion were determined in transfected L cell fibroblasts. These parameters were measured using the nonesterifiable fluorescent fatty acid probe 12- N-methyl-(7-nitrobenz-2-oxa-1,3-diazol)aminostearate (NBD-stearate) and fluorescence digital imaging. In single-cell fluorescence imaging experiments, L-FABP-expressing cells, but not I-FABP-expressing cells, increased NBD-stearate uptake 1.7-fold compared with control cells. Both I- and L-FABP increased the cytoplasmic diffusion rate of the internalized NBD-stearate 2.6- and 1.9-fold, respectively, compared with control cells. However, increased NBD-stearate lateral membrane mobility was observed only in L-FABP-expressing cells. After incubation of the cells with 4 μM NBD-stearate at 37°C for 30 min, fluorescence deconvolution imaging indicated that NBD-stearate was localized primarily into lipid droplets in all cell lines. The differential effect of these proteins on fatty acid uptake and intracellular trafficking in single cells illustrates a possible difference in the physiological function of I- and L-FABP in intact cells. |
| Starting Page | G244 |
| Ending Page | G249 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajpgi.physiology.org/content/ajpgi/275/2/G244.full.pdf |
| PubMed reference number | 29585534v1 |
| Alternate Webpage(s) | https://doi.org/10.1152/ajpgi.1998.275.2.G244 |
| DOI | 10.1152/ajpgi.1998.275.2.g244 |
| Journal | American journal of physiology. Gastrointestinal and liver physiology |
| Volume Number | 275 |
| Issue Number | 2 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aluminum stearate Cultured Cell Line FABP1 protein, human Fabp5 protein, rat Fatty Acids Fluorescence Imaging GLYCERYL STEARATE SE Leukemia, B-Cell Lipid Metabolism Disorders Nucleosome Binding Domain Stearates Thioctic Acid Tissue membrane |
| Content Type | Text |
| Resource Type | Article |
