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The Synthesis of Tritium-labeled 14α-Methyl-5α-cholest-7-en-3β-ol and Its Enzymatic Demethylation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Knight, John Cantey Klein, Peter Dr. Szczepanik, Patricia A. |
| Copyright Year | 1966 |
| Abstract | Abstract 14α-Methyl-5α-cholest-7-en-3-one has been prepared and labeled with tritium by isotope exchange chromatography. Reduction with lithium aluminum hydride furnished pure, highly labeled 14α-methyl-5α-cholest-7-en-3β-ol. Incubation of this compound with homogenates of rat liver results in its conversion to cholesterol, which has been identified by a variety of chemical and chromatographic techniques. The results show that enzymatic removal of the 14α-methyl group from synthetic compounds is feasible, and offer a new approach to the study of the demethylation mechanism in steroid structures. |
| Starting Page | 1502 |
| Ending Page | 1508 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Volume Number | 241 |
| Alternate Webpage(s) | http://www.jbc.org/content/241/7/1502.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |