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Sequencing of the large dsDNA genome of Oryctes rhinoceros nudivirus using multiple displacement amplification of nanogram amounts of virus DNA.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Yongjie Kleespies, Regina G. Ramle, M. Jehle, Johannes A. |
| Copyright Year | 2008 |
| Abstract | The genomic sequence analysis of many large dsDNA viruses is hampered by the lack of enough sample materials. Here, we report a whole genome amplification of the Oryctes rhinoceros nudivirus (OrNV) isolate Ma07 starting from as few as about 10 ng of purified viral DNA by application of phi29 DNA polymerase- and exonuclease-resistant random hexamer-based multiple displacement amplification (MDA) method. About 60 microg of high molecular weight DNA with fragment sizes of up to 25 kbp was amplified. A genomic DNA clone library was generated using the product DNA. After 8-fold sequencing coverage, the 127,615 bp of OrNV whole genome was sequenced successfully. The results demonstrate that the MDA-based whole genome amplification enables rapid access to genomic information from exiguous virus samples. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.jviromet.2008.06.003 |
| PubMed reference number | 18598718 |
| Journal | Medline |
| Volume Number | 152 |
| Issue Number | 1-2 |
| Alternate Webpage(s) | https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00020819/2008-0007.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.jviromet.2008.06.003 |
| Journal | Journal of virological methods |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
