Refolding of β-galactosidase: microfluidic device for reagent metering and mixing and quantification of refolding yield

Content Provider	Semantic Scholar
Author	Kondapalli, Sowmya Kirby, Brian J.
Copyright Year	2009
Abstract	We have developed a device that uses microfluidic valves and pumps to meter reagents for subsequent mixing with application to refolding of the protein β-galactosidase. The microfluidic approach offers the potential advantages of automation, cost-effectiveness, compatibility with optical detection, and reduction in sample volumes as opposed to conventional techniques of hand-pipetting or using robotic systems. The device is a multi-layered poly(dimethylsiloxane) on glass device with automated controls for reagent aliquoting and mixing. Refolding experiments have been performed off-chip using existing protocols on the protein β-galactosidase and the refolding yield has been quantified on-chip using fluorescein di-β-d-galactopyranoside, a caged-fluorescent molecule. This work provides the potential to reduce the cost of drug discovery and realization of protein pharmaceuticals.
Starting Page	275
Ending Page	281
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1007/s10404-008-0388-z
Volume Number	7
Alternate Webpage(s)	http://www.kirbyresearch.com/pdf/200908kondapalli.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s10404-008-0388-z
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article