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In vivo efflux of serotonin in the dorsal raphe nucleus of 5-HT1A receptor knockout mice.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bortolozzi, Analía Amargós-Bosch, Mercè Tóth, Miklós Artigas, Francesc Casañas Adell, Albert |
| Copyright Year | 2004 |
| Abstract | In the dorsal raphe nucleus (DR), extracellular serotonin (5-HT) regulates serotonergic transmission through 5-HT1A autoreceptors. In this work we used in vivo microdialysis to examine the effects of stressful and pharmacological challenges on DR 5-HT efflux in 5-HT1A receptor knockout (5-HT1A-/-) mice and their wild-type counterparts (5-HT1A+/+). Baseline 5-HT concentrations did not differ between both lines of mice, which is consistent with a lack of tonic control of 5-HT1A autoreceptors on DR 5-HT release. (R)-(+)-8-Hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8-OH-DPAT, 0.5 mg/kg) reduced 5-HT levels to 30% of basal values in 5-HT1A+/+ mice, but not in 5-HT1A-/- mice. The selective 5-HT1B receptor agonist 1,4-dihydro-3-(1,2,3,6-tetrahydro-4-pyridinyl)-5H-pyrrolo[3,2-b]pyridin-5-one dihydrochloride (CP 93129, 300 micro m) reduced dialysate 5-HT to the same extent (30-40% of baseline) in the two genotypes, which suggests a lack of compensatory changes in 5-HT1B receptors in the DR of such mutant mice. Both a saline injection and handling for 3 min increased DR dialysate 5-HT in mutants, but not in 5-HT1A+/+ mice. Fluoxetine (5 and 20 mg/kg) elevated 5-HT in a dose-dependent manner in both genotypes. However, this effect was markedly more pronounced in the 5-HT1A-/- mice. The increased responsiveness of the extracellular 5-HT in the DR of 5-HT1A receptor knockout mice reflects a lack of the autoinhibitory control exerted by 5-HT1A autoreceptors. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://digital.csic.es/bitstream/10261/33047/1/j.1471-4159.2003.02267.x.pdf |
| PubMed reference number | 15009637v1 |
| Volume Number | 88 |
| Issue Number | 6 |
| Journal | Journal of neurochemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 5-Hydroxytryptamine Receptor 1A, human Autoreceptors Cell Nucleus Dialysate - Specimen Source Codes Dialysis Solutions Fluoxetine Genotype HTR1A wt Allele Handling (Psychology) Microdialysis Pharmacology Pramipexole dihydrochloride 0.5 MG Oral Tablet Receptor, Serotonin, 5-HT1A Receptor, Serotonin, 5-HT1B Serotonin dorsal raphe nucleus mg/kg mutant physical hard work raphe nuclei |
| Content Type | Text |
| Resource Type | Article |
