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Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Delmotte, Francis M. Goldstein, Irwin J. |
| Copyright Year | 1980 |
| Abstract | Bandeiraea simplicifolia plant seeds contain a family of five alpha-D-galactopyranosyl-binding isolectins (BS I-A4, A3B, A2B2, AB3, B4) and N-acetyl-D-glucosamine-binding lectin (BS II). After Pi/NaCl extraction and ammonium sulfate fractionation BS II is specifically adsorbed onto p-aminobenzyl-1-thio-N-acetyl-beta-D-glucosaminide-succinylaminohexylaminyl--Sepharose-4B. The BS I isolectins pass through this column and BS II is selectively eluted by Pi/NaCl containing 2 mM N-acetyl-D-glucosamine or by 0.1 M sodium acetate buffer pH 3.6. The material not bound to the column is loaded onto p-aminophenyl-beta-D-galactopyranosyl-succinylaminohexylaminyl--Sepharose-4B. BS I-A4 is specifically eluted in a sharp peak with Pi/NaCl containing 1 mM N-acetyl-D-galactosamine. Then, BS I-A3B, A2B2, AB3 and B4 are selectively eluted, in a single peak for each isolectin. with Pi/NaCl containing 2 mM, 8 mM, 15 mM and 50 mM methyl alpha-D-galactopyranoside, respectively. |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1432-1033.1980.tb07197.x |
| Alternate Webpage(s) | https://deepblue.lib.umich.edu/bitstream/handle/2027.42/66350/j.1432-1033.1980.tb07197.x.pdf?sequence=1 |
| PubMed reference number | 7460921 |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1432-1033.1980.tb07197.x |
| Journal | Medline |
| Volume Number | 112 |
| Issue Number | 2 |
| Journal | European journal of biochemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
