MOLECULAR VARIABILITY IN ISOLATES OF Rhizoctonia bataticola CAUSING ROOT ROT IN CHICKPEA.

Content Provider	Semantic Scholar
Author	Walunj, Puja Maruti.
Copyright Year	2018
Abstract	The Genetic diversity of all the isolates of Rhizoctonia bataticola was analysed by using Inter-Simple Sequence Repeat (ISSR) markers. The ISSR analysis, a total 10 primers were screened against eight isolates of Rhizoctonia bataticola. Out of 10 primers 9 primers amplified scorable banding pattern. Out of 37 bands, 30 bands were polymorphic and 7 bands are monomorphic. Average level of Polymorphism was 81.08%. In the dendrogram, Rb-2 (Nagpur) was found to have higher value of similarity coefficient (0.808) whereas Rb-1(Akola) was found to have lower value of similarity coefficient (0.375). The isolates of Rhizoctonia bataticola were grouped into four major clusters. First group is named as clusterA, includes isolates belonging to Akola, Amravati and Sangli. Second group is named as cluster-B, includes Nagpur, Karanje and Kini, third group is named as cluster-C, includes Umbraj and fourth grouped is named as cluster-D includes Kurundwad. It shows that Rb-2 (Nagpur) have higher value of similarity coefficient with Rb-5 (Karanje) whereas Rb-1 (Akola) have lower value of similarity coefficient with Rb-8 (Kini). The similarity matrix indicated that eight isolates of Rhizoctonia bataticola exhibited (37.5 to 80.8) percent similarity coefficient.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.chemijournal.com/archives/2018/vol6issue5/PartBG/6-5-292-898.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article