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Lipase-catalyzed interesterification of soybean oil with an omega-3 polyunsaturated fatty acid concentrate prepared from sardine oil.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Akimoto, Masamichi Izawa, Maki Hoshino, Kazumi Abe, Ken-Ichi Takahashi, Hiromi |
| Copyright Year | 2003 |
| Abstract | To reduce the content of linoleoyl moiety in soybean oil, soybean oil that contains 53.0% linoleoyl moiety as molar acyl moiety composition was interesterified with an omega-3 polyunsaturated fatty acid (PUFA) concentrate (24.0 mol% eicosapentaenoic acid [EPA], 40.4 mol% docosahexaenoic acid [DHA]) prepared from sardine oil, using an immobilized sn-1,3-specific lipase from Rhizomucor miehei (Lipozyme IM). The reaction was carried out in a batch reactor at 37 degrees C under the following conditions: 500 micromol of soybean oil, molar ratio of omega-3 PUFA concentrate to soybean oil = 1.0-6.0,5.0 mL of heptane, and 30 batch interesterification units of enzyme. After the reaction time of 72 h, modified soybean oil, which contains 34.9% linoleoyl, 10.1% eicosapentaenoyl, and 14.2% docosahexaenoyl moieties, was produced at the molar reactant ratio of 6.0. In this oil, the total omega-3 acyl moiety composition reached 34.1%; the molar ratio of omega-3 to omega-6 acyl moieties was enhanced by five times compared with soybean oil. Compared with palmitic acid, DHA was kinetically six times less reactive, although the EPA was by 16% more reactive. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1385/ABAB:104:2:105 |
| PubMed reference number | 12603099v1 |
| Volume Number | 104 |
| Issue Number | 2 |
| Journal | Applied biochemistry and biotechnology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Eicosapentaenoic Acid Heptanes Lipase Lipozyme Micromole Omega-3 Fatty Acids Palmitic Acid Polyunsaturated Fatty Acids Reactor Device Component Rhizomucor pusillus Ab:PrThr:Pt:Ser:Ord Soybean Oil TIMP1 protein, human docosahexaenoic acid linoleoyl-CoA desaturase |
| Content Type | Text |
| Resource Type | Article |
