Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway.

Content Provider	Semantic Scholar
Author	Yonezawa, Tomo Haga, Satoshi Kobayashi, Yosuke Takahashi, Tatsuyuki Obara, Yoshiaki
Copyright Year	2006
Abstract	Visfatin was originally identified as a growth factor for immature B cells, and recently demonstrated to bind insulin receptor. Visfatin mRNA and protein were detected by RT-PCR and Western blot analysis in cloned bovine mammary epithelial cells, lactating bovine mammary gland and human breast cancer cell line, MCF-7. Immunocytochemical staining localized the visfatin protein in the cytosol and nucleus of both cells. Quantitative-RT-PCR analysis revealed that the expression of the visfatin mRNA was significantly elevated when treated with forskolin (500 microM), isopreterenol (1-10 microM) and dibutyric cyclic AMP (1 mM) for 24 h, and significantly reduced when treated with insulin (5-50 ng/ml) and dexsamethasone (0.5-250 nM) for 24 h. These results indicate that mammary epithelial cells express the visfatin protein and secrete them into the milk.
File Format	PDF HTM / HTML
DOI	10.1016/j.febslet.2006.11.014
Alternate Webpage(s)	https://core.ac.uk/download/pdf/82535497.pdf
PubMed reference number	17123517
Alternate Webpage(s)	https://doi.org/10.1016/j.febslet.2006.11.014
Journal	Medline
Volume Number	580
Issue Number	28-29
Journal	FEBS letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article