NDLI: Adherence of human basophils to cultured umbilical vein endothelial cells.

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Adherence of human basophils to cultured umbilical vein endothelial cells.

Content Provider	Semantic Scholar
Author	Bochner, Bruce S. Peachell, P. T. Brown, Kyle E. Schleimer, Robert P.
Copyright Year	1988
Abstract	The mechanism by which circulating human basophils adhere to vascular endothelium and migrate to sites of allergic reactions is unknown. Agents have been identified which stimulate the adherence of purified basophils to cultured human umbilical vein vascular endothelial cells (HuVEC). Treatment of HuVEC with interleukin 1, tumor necrosis factor (TNF), bacterial endotoxin, and 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in time and dose-dependent increases of adhesiveness for basophils. Coincubation of basophils and HuVEC for 10 min with C5a, formyl-methionyl-leucyl-phenylalanine, the calcium ionophore A23187, platelet-activating factor, TNF, and TPA also resulted in significant dose-dependent increases in basophil adherence; this effect resulted from activation of the basophil. Adherence of basophils to HuVEC was time and temperature dependent, required divalent cations, and was unaffected by glucocorticoids. Monoclonal antibody 60.3, directed against the beta-subunit of the leukocyte adherence complex CD18, inhibited the binding of basophils to HuVEC. Adherence of basophils to vascular endothelium may be important in initiating basophil infiltrates in vivo.
Starting Page	2226
Ending Page	2230
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://dm5migu4zj3pb.cloudfront.net/manuscripts/113000/113463/JCI88113463.pdf
PubMed reference number	3130394v1
Volume Number	81
Issue Number	5
Journal	The Journal of clinical investigation
Language	English
Access Restriction	Open
Subject Keyword	Adhesiveness Basophils Blood Platelets CD18 Antigens Calcium Cations Cations, Divalent Complement C5a Drug Allergy Endothelium, Vascular Endotoxins Infiltration Interleukins Ionophores Leukocytes Melphalan Platelet Activating Factor Tetradecanoylphorbol Acetate Total Basophil Count Tumor Necrosis Factors Umbilical vein Umbilicus (Anatomy) trastuzumab
Content Type	Text
Resource Type	Article

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