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Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liao, David Galajda, Péter Riehn, Robert Ilic, Rob Puchalla, Jason L. Yu, Howard Craighead, Harold G. Austin, Robert H. |
| Copyright Year | 2008 |
| Abstract | Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to approximately 17 mm/s. We derive an analytic scaling relationship [equation: see text] converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow. |
| File Format | PDF HTM / HTML |
| DOI | 10.1364/OE.16.010077 |
| PubMed reference number | 18607415 |
| Journal | Medline |
| Volume Number | 16 |
| Issue Number | 14 |
| Alternate Webpage(s) | http://www.cnf.cornell.edu/doc/2008cnfRApdfs/2008cnfRApg10.pdf |
| Alternate Webpage(s) | https://doi.org/10.1364/OE.16.010077 |
| Journal | Optics express |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |