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Oxidative response of Vitis vinifera cells elicited with Phaeomoniella chlamydospora
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lima, Marta Maria Rodrigues Dias, Alberto Carlos Pires |
| Copyright Year | 2007 |
| Abstract | • A first experiment was made to observe de oxidative response of Vv cell cultures to Pc extract elicitation; cultures were divided into 2 groups: a control group and a group elicited with Pc extract (0,5 mg/ml). • In a second experiment we studied the relationship between Ca signalling and oxidative burst; cultures were elicited with Pc extract (0,5 mg/ml), EGTA (5mM), LaCl3 (100 μM), nifedipine (100 μM) and ruthenium red (100 μM), as well as primed with EGTA 20 minutes prior to Pc elicitation and with nifedipine, ruthenium red and LaCl3 30 seconds prior to Pc elicitation (using the above concentrations). • In a third experiment we assayed the involvement of NADPHoxidase in the oxidative burst: cultures were elicited with Pc extract (0,5 mg/ml) and DPI (10 μM) as well as primed with DPI 10 minutes before Pc elicitation (using the above concentrations). • In every experiment elicitation occurred on the 6th day of culture and samples were taken during the first 7 hours after Pc elicitation. Elicitation and sample collection |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorium.sdum.uminho.pt/bitstream/1822/9316/1/Ghent2007.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
