NDLI: Expression of hepatitis B surface antigen (HBsAg) gene in transgenic cherry tomato

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Expression of hepatitis B surface antigen (HBsAg) gene in transgenic cherry tomato

Content Provider	Semantic Scholar
Author	Guan, Zheng-Jun Hao, Hao-Yong Huo, Yan-Lin Dai, Jia-Kun Wei, Ya-Hui
Copyright Year	2012
Abstract	The plasmid carrying the gene (adr subtype) encoding the hepatitis B surface antigen (HBsAg) was constructed. Cotyledonary leaves of cherry tomato (Lycopersicon esculentum Mill.) were transformed using Agrobacterium tumefaciens strain LBA4404 harboring pCAMBIA-1301/HB constructs to express HBsAg. The transformed nature of the plants was confirmed by polymerase chain reaction (PCR) analysis and Southern blot hybridization. Expression of HBsAg was confirmed by western blotting and levels of expression were assayed by enzyme-linked immunosorbent assay (ELISA). Southern blot hybridization confirmed the stable integration of the target genes into the genomes of cherry tomato, while western blotting showed high levels of biologically active HBsAg in transgenic plants. ELISA assay showed that HBsAg in transgenic cherry tomato was 100.36 ng/g fresh weight (FW) in leaves and 127.54 ng/g FW in fruits, implying that recombinant HBsAg had natural epitope. This study indicates the feasibility of the expression of foreign antigens in cherry tomato plant for possible use as a raw and edible vaccine.
Starting Page	7186
Ending Page	7192
Page Count	7
File Format	PDF HTM / HTML
Volume Number	11
Alternate Webpage(s)	https://www.ajol.info/index.php/ajb/article/download/102252/92289
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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