Gadolinium triggers unfolded protein responses (UPRs) in primary cultured rat cortical astrocytes via promotion of an influx of extracellular Ca2+

Content Provider	Semantic Scholar
Author	Feng, Xu-Dong Xia, Qing Yuan, Lan Huang, Hai-Feng Yang, Xiaoda Wang, Kui
Copyright Year	2010
Abstract	Gadolinium (Gd) and its complexes are utilized widely in industrial and clinical diagnoses. As a rare earth metal ion, free gadolinium (Gd3+) in the human body poses neurotoxic risks during its in vivo release and retention. In the central nervous system, astrocytes play a pivotal role in processing toxic metal ions. The present study evaluates the effects of Gd on cellular calcium homeostasis, a common mechanism that causes cell death, and on unfolded protein responses (UPRs), a mechanism for cell survival in response to toxic stimuli in mammalian cells. The experimental results indicate that the influx of extracellular Ca2+ increases greatly after the exposure of astrocytes to Gd; however, no cell deaths were observed. Further evidence suggests the up-regulated expression of the endoplasmic reticulum (ER)-resident chaperone protein GRP78 by ER stress-mediated signal transductions, specifically the activation of ATF6, eIF2a, and IRE1. These results suggest that Gd promotes Ca2+ influx, thus triggering UPRs, which can be closely associated to the resistance of astrocytes to Gd-induced cytotoxicity.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1007/s10565-010-9166-2
PubMed reference number	20419501
Journal	Medline
Volume Number	27
Alternate Webpage(s)	http://or.nsfc.gov.cn/bitstream/00001903-5/21488/1/1000002685622.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s10565-010-9166-2
Journal	Cell Biology and Toxicology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article