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Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage
| Content Provider | Semantic Scholar |
|---|---|
| Author | Khamidakh, A. E. Abu Rodriguez-Martinez, Alejandra Kaarniranta, Kai Kallioniemi, Anne Skottman, Heli Hyttinen, Jari Juuti-Uusitalo, Kati |
| Copyright Year | 2018 |
| Abstract | BackgroundWound healing of retinal pigment epithelium (RPE) is a complex process that may take place in common age-related macular degeneration eye disease. The purpose of this study was to evaluate whether wounding and wound healing has an effect on Ca2+ dynamics in human embryonic stem cell (hESC)-RPEs cultured different periods of time.MethodsThe 9-day-cultured or 28-day-cultured hESC-RPEs from two different cell lines were wounded and the dynamics of spontaneous and mechanically induced intracellular Ca2+ activity was measured with live-cell Ca2+ imaging either immediately or 7 days after wounding. The healing time and speed were analyzed with time-lapse bright field microscopy. The Ca2+ activity and healing speed were analysed with image analysis. In addition the extracellular matrix deposition was assessed with confocal microscopy.Results The Ca2+ dynamics in hESC-RPE monolayers differed depending on the culture time: 9-day-cultured cells had higher number of cells with spontaneous Ca2+ activity close to freshly wounded edge compared to control areas, whereas in 28-day-cultured cells there was no difference in wounded and control areas. The 28-day-cultured, wounded and 7-day-healed hESC-RPEs produced wide-spreading intercellular Ca2+ waves upon mechanical stimulation, while in controls propagation was restricted. Most importantly, both wave spreading and spontaneous Ca2+ activity of cells within the healed area, as well as the cell morphology of 28-day-cultured, wounded and thereafter 7-day-healed areas resembled the 9-day-cultured hESC-RPEs.ConclusionsThis acquired knowledge about Ca2+ dynamics of wounded hESC-RPE monolayers is important for understanding the dynamics of RPE wound healing, and could offer a reliable functionality test for RPE cells. The data presented in here suggests that assessment of Ca2+ dynamics analysed with image analysis could be used as a reliable non-invasive functionality test for RPE cells. |
| File Format | PDF HTM / HTML |
| DOI | 10.1186/s12938-018-0535-z |
| PubMed reference number | 30064430 |
| Journal | Medline |
| Volume Number | 17 |
| Alternate Webpage(s) | https://tutcris.tut.fi/portal/files/16376300/full_text.pdf |
| Alternate Webpage(s) | http://tampub.uta.fi/bitstream/handle/10024/104057/Wound_healing_of_human_embryonic_stem_cell-derived_retinal_pigment_2018.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://biomedical-engineering-online.biomedcentral.com/track/pdf/10.1186/s12938-018-0535-z |
| Alternate Webpage(s) | https://erepo.uef.fi/bitstream/handle/123456789/6853/15351101031181811943.pdf?isAllowed=y&sequence=2 |
| Alternate Webpage(s) | https://doi.org/10.1186/s12938-018-0535-z |
| Journal | Biomedical engineering online |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
