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Thyroid hormones in blood and milk of lactating donkey: preliminary results
| Content Provider | Semantic Scholar |
|---|---|
| Author | Todini, Luca Fantuz, Francesco Malfatti, Alessandro Brunetti, Valeria L. Salimei, Elisabetta |
| Copyright Year | 2010 |
| Abstract | MATERIALS AND METHODS 16 lactating jennies, 32-58 days postpartum at the beginning of the study, stabled with their foals and machine milked after a 3 h-separation Feeding: 8 kg of coarse hay and 2.5 kg of mixed feed (12.8 % CP) daily Samplings at 14 d-interval, milk samples immediately processed for iodothyronines extraction with alkaline ethanol at – 20°C, blood samples collected in evacuated tubes (K3-EDTA), immediately centrifuged and the plasmas stored at −20°C until assayed Total concentrations of T3 in milk and T3 and T4 in plasma were assayed using EIA kits (Radim, Rome, Italy), expressly validated for donkey species (intraand inter-assay CVs for T3 and T4 and for milk and plasma: 2-8%). ERI LS E S 16 lactating jennies, 32-58 days postpartu at the beginning of the study, stabled with their foals and achine ilked after a 3 h-separation Feeding: 8 kg of coarse hay and 2.5 kg of ixed feed (12.8 CP) daily Sa plings at 14 d-interval, ilk sa ples i ediately processed for iodothyronines extraction with alkaline ethanol at – 20°C, blood sa ples collected in evacuated tubes (K3-E T ), i ediately centrifuged and the plas as stored at −20°C until assayed Total concentrations of T3 in ilk and T3 and T4 in plas a were assayed using EI kits (Radi , Ro e, Italy), expressly validated for donkey species (intraand inter-assay CVs for T3 and T4 and for ilk and plas a: 2-8 ). |
| Starting Page | 43 |
| Ending Page | 43 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://old.eaap.org/Previous_Annual_Meetings/2010Crete/Papers/06_Todini.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
