Loading...
Please wait, while we are loading the content...
Similar Documents
Analysis of Broad-Range DNA Fragments with Yttrium Oxide or Ytterbium Oxide Nanoparticle/Polymer Sieving Matrix Using High-Performance Capillary Electrophoresis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kwon, Haemyun Kim, Yong-Seong |
| Copyright Year | 2009 |
| Abstract | plates/m) and the baseline resolution for various DNA standards (100 bp, 500 bp, and 1 kbp DNA ladder) were obtained in 10 min with these matrices. The effects of concentrations of both polyethylene oxide (PEO) and nanoparticles were investigated and the highest performance was obtained at 0.02% PEO with 0.02% YNP or YbNP. Similar sieving power for both YNP and YbNP matrices was observed probably due to the similar sizes of nanoparticles, resulting in the formation of comparable sieving networks for DNA separation. For the reduction of electrosmotic flow, either dynamic or permanent coating of the capillary inner wall was compared and it turned out that PEO was superior to polyvinylpyrrolidone (PVP) or polyacrylamide (PAA) for better separation efficiency. |
| Starting Page | 297 |
| Ending Page | 301 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.5012/bkcs.2009.30.2.297 |
| Volume Number | 30 |
| Alternate Webpage(s) | http://ocean.kisti.re.kr/downfile/volume/chemical/JCGMCS/2009/v30n2/JCGMCS_2009_v30n2_297.pdf |
| Alternate Webpage(s) | https://doi.org/10.5012/bkcs.2009.30.2.297 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |