NDLI: Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting.

Content Provider	Semantic Scholar
Author	Johnson, Lawrence A. Flook, J. P. Hawk, H. W.
Copyright Year	1989
Abstract	Intact, viable X and Y chromosome-bearing sperm populations of the rabbit were separated according to DNA content with a flow cytometer/cell sorter. Reanalysis for DNA of an aliquot from each sorted population showed purities of 86% for X-bearing sperm and 81% for Y-bearing sperm populations. Sorted sperm were surgically inseminated into the uterus of rabbits. From does inseminated with sorted X-bearing sperm, 94% of the offspring born were females. From does inseminated with sorted Y-bearing sperm from the same ejaculates, 81% of the offspring were males. The probability of the phenotypic sex ratios differing from 50:50 were p less than 0.0003 for X-sorted sperm and p less than 0.004 for Y-sorted sperm. Thus, the phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.
Starting Page	199
Ending Page	203
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1095/biolreprod41.2.199
PubMed reference number	2804212
Journal	Medline
Volume Number	41
Issue Number	2
Alternate Webpage(s)	https://watermark.silverchair.com/biolreprod0199.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAjMwggIvBgkqhkiG9w0BBwagggIgMIICHAIBADCCAhUGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMclXpRI4qBaYhxwZGAgEQgIIB5jTnZFyD1zXrbQPIA69zLmF2CH_e5S2d_8EBfiXDFlH9sZ-jQcxFB18hBxj31JC98y4p2ryfEryPwd2SlN1YfWHPlcVatKBIKfcuNTjEp5voZiuuAQY8MZjQP73Ox3feSfMeyfS40n-lR88NSclR1tjaL3cYv__floy1VFiI7F5FDhzE7BAcbwbOp_zoEBRwU4HfdjQYuzOYUfJuXyN80KVgCZaOzpKLA4IMATX3ggmkPkLk3B2gms_HaYa6op8eWIC_RvmZRTuLK2dERK5bRBRPFBNrlRxCbTV5xBakRNaBXr3VCsU2tOmvxYEqRKmJO4dii9mmPenrFa2vJt1A-E6uHL4VswmkV3AwPrgF6qXQsL4PGQxpD2m2KSNiyVy_Z0JYqhbosKoYufCBpXYDr4hDtuGqTKXNOXrfBqzC3pvu8nfho8E9WfAdUxD8iZ__eFbIYW7rokyPrq8-39giWpEyOOz2bWf-Pv4f3WIddQqdY-rof9Axwb4PGKMiz3NuMOEf8Xu_fKnLkHu0wD6WC5KuagEl1rb9X-6oE-VrE0oPz_9TC1WASsmPvqD-a__Y4u8eQohvtz65aYTr8otA-RZAWPldka1589gbcLJMSaX4U_jL9BMyFveV-ZgX5MrO_YANVvP8tQ
Alternate Webpage(s)	https://doi.org/10.1095/biolreprod41.2.199
Journal	Biology of reproduction
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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