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Lack of significant changes in μ, δ opioid binding sites and neutral endopeptidase EC 3.4.24.11 in the brain and spinal cord of arthritic rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Delay-Goyet, Philippe Kayser, Valérie Guilbaud, G. Roques, Bernard Pierre |
| Copyright Year | 1989 |
| Abstract | Abstract The possible changes in neutral endopeptidase EC 3.4.24.11 (“enkephalinase”, NEP), μ and δ opioid binding sites, were investigated using in vitro quantitative radioautography in various regions of the central nervous system of the Freund's adjuvant-induced arthritic rat, a model of chronic pain. Enkephalinase was labelled by a specific tritiated inhibitor, [ 3 H ]N-[(2 RS )-3- hydroxyaminocarbonyl -2- benzyl-l-oxopropyl]glycine ([ 3 H]HACBO-Gly), while μ and δ opioid binding sites were selectively labelled with [ 3 H]Tyr- d -Ala-Gly-(Me)Phe-Gly-ol ([ 3 H]DAGO) and [ 3 H]Tyr- d -Thr-Gly-Phe-Leu-Thr ([ 3 H]DTLET), respectively. As compared to controls, no significant modifications were found in NEP, μ or δ binding sites at both supraspinal and spinal levels of arthritic rats. These results suggest that the enhanced efficiency of exogenous opioids or endogenous enkephalins, reported to occur in this model of chronic inflammatory pain, are not directly related to changes in μ and δ opioid binding sites or steady state levels of NEP. |
| Starting Page | 1341 |
| Ending Page | 1348 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0028-3908(89)90008-7 |
| Volume Number | 28 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0028390889900087 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0028390889900087?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0028-3908%2889%2990008-7 |
| Journal | Neuropharmacology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
