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Dual targeting improves microbubble contrast agent adhesion to VCAM-1 and P-selectin under flow.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ferrante, Elisa A. Pickard, John E. Rychak, J. Klibanov, A. Ley, Klaus |
| Copyright Year | 2009 |
| Abstract | To improve ultrasound contrast agents targeted to the adhesion molecules P-selectin and VCAM-1 for the purpose of molecular imaging of atherosclerotic plaques, perfluorocarbon-filled phospholipid microbubble contrast agents were coupled by a polyethylene glycol-biotin-streptavidin bridge with mAb MVCAM.A(429), a sialyl Lewis(x) polymer (PAA-sLe(x)), or both (dual). Approximately three hundred thousand antibody molecules were coupled to the surface of each microbubble. Recombinant mouse P-selectin and/or VCAM-1 coated on flow chambers showed saturation of binding at approximately 15 ng/microl, resulting in 800 and 1200 molecules/microm(2) for P-selectin and VCAM-1, respectively. Dual substrates coated with equal concentrations of P-selectin and VCAM-1 had site densities between 50 and 60% of single substrates. When microbubbles were perfused through flow chambers at 5 x 10(6) microbubbles/ml (wall shear stress from 1.5 to 6 dyn/cm(2)) dual-targeted microbubbles adhered almost twice as efficiently as single-targeted microbubbles at 6 dyn/cm(2). The present study suggests that dual-targeted contrast agents may be useful for atherosclerotic plaque detection at physiologically relevant shear stresses. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.jconrel.2009.08.001 |
| PubMed reference number | 19666063 |
| Journal | Medline |
| Volume Number | 140 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://ley-lab.liai.org/publications/294.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.jconrel.2009.08.001 |
| Journal | Journal of controlled release : official journal of the Controlled Release Society |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
