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Caracterisation des proteines de granules denses de toxoplasma gondii : Etude des interactions protéiques et lipidiques et du rôle des hélices alpha-amphipatiques de GRA2
| Content Provider | Semantic Scholar |
|---|---|
| Author | Travier, Laetitia |
| Copyright Year | 2007 |
| Abstract | At the interface with the host cell compartment, the parasitophorous vacuole of the intracellular parasite Toxoplasma gondii comprises several membranous systems, including the membranous nanotubular network formed upon secretion of the dense granule protein GRA2. Using biochemical approaches, I have shown that the GRA proteins form oligomeric complexes, which could explain the solubility of these membrane proteins within the dense granules and after secretion into the vacuolar soluble fraction. Using molecular and cellular approaches, I have investigated the involvement of each of the three GRA2 amphipathic alpha-helices in post-secretory membrane association of the protein, likely via association to phosphatidylinositol phosphates, and in the GRA2 induced-formation of the membranous nanotubular network. These studies led us to propose models for the interaction of GRA2 with membranes and for the tubulation of the membranous nanotubular network. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://tel.archives-ouvertes.fr/tel-00182730/document |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |