NDLI: Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography.

Content Provider	Semantic Scholar
Author	Maurya, Anupam Srivastava, Santosh Kumar
Copyright Year	2009
Abstract	Centrifugal partition chromatography in the pH-zone-refining mode was successfully applied to the separation of alkaloids, directly from a crude extract of Ipomoea muricata. The experiment was performed with a two-phase solvent system composed of methyl tert-butyl ether (MtBE)-acetonitrile-water (4:1:5, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and trifluoroacetic acid (10 mM) to the aqueous mobile phase as an eluter. From 4 g of crude extract, 210 mg lysergol and 182 mg chanoclavine were obtained in 97% and 79.6% purities. Total yield recovery was >95%. Isolated alkaloids were characterized on the basis of their (1)H, (13)C NMR and ESI-MS data.
File Format	PDF HTM / HTML
DOI	10.1016/j.jchromb.2009.04.036
Alternate Webpage(s)	http://staff.cimap.res.in/PublicationFiles%5CJ._Chromatogr._B_877_1732%E2%80%931736.pdf
PubMed reference number	19467935
Alternate Webpage(s)	https://doi.org/10.1016/j.jchromb.2009.04.036
Journal	Medline
Volume Number	877
Issue Number	18-19
Journal	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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