NDLI: Phenotypic profiles and detection of target genes by PCR in isolates from different sources and reference strains, identified as Cronobacter spp. (Enterobacter sakazakii)

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Phenotypic profiles and detection of target genes by PCR in isolates from different sources and reference strains, identified as Cronobacter spp. (Enterobacter sakazakii)

Content Provider	Semantic Scholar
Author	Warnken, Márcia Barbosa Brandão, Marcelo Luiz Lima Souza, Aline Encarnação Romão, Célia Maria Carvalho Pereira Araulo Nogueira, Ana Cristina Martins Destro, Maria Teresa
Copyright Year	2012
Abstract	Cronobacter, formerly known as Enterobacter sakazakii, is a novel genus of the Enterobacteriaceae family recognized as a cause of high number of fatal cases in neonates, after consuming infant formula. The conventional methods for detecting these organisms are time-consuming and lack sensitivity. The ISO/TS 22964:2006 is the most recently standardized methodology for detecting Cronobacter in powdered infant formula. This study aimed at confirming the Brazilian isolates previously identified as E. sakazakiias Cronobacter spp. by biochemical assays, and also to compare characteristics of 37 Cronobacter andnon-Cronobacter isolates; and the miniaturized kits and the ISO/TS methodology were evaluated. Aconventional PCR protocol targeting dnaG was also developed and a previously described gluA targeting protocol was used. The majority of the Brazilian isolates were not confirmed as Cronobacter spp., and the selective enrichment step of ISO/TS methodology was inhibitory to some Cronobacter strains. The ID 32E was the most reliable kit. The PCR protocol targeting gluA showed consistent results with ID 32E and the developed dnaG PCR protocol was 100% sensitive and specific. Thus, the PCR protocols targeting gluAand dnaG might be used to complement the Cronobacter spp. detection or identification after performing the conventional isolation and identification methods.
Starting Page	21
Ending Page	31
Page Count	11
File Format	PDF HTM / HTML
Volume Number	71
Alternate Webpage(s)	http://www.ial.sp.gov.br/resources/insituto-adolfo-lutz/publicacoes/rial/10/rial71_1_completa/1428.pdf
Alternate Webpage(s)	https://www.arca.fiocruz.br/bitstream/icict/9190/2/RIAL_71_21-31.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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