NDLI: Characterization of an Alkaline GH49 Dextranase from Marine Bacterium Arthrobacter oxydans KQ11 and Its Application in the Preparation of Isomalto-Oligosaccharide

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Characterization of an Alkaline GH49 Dextranase from Marine Bacterium Arthrobacter oxydans KQ11 and Its Application in the Preparation of Isomalto-Oligosaccharide

Content Provider	Semantic Scholar
Author	Liu, Hongfei Ly, Mingsheng Li, Hai-Feng Wang, Shujun
Copyright Year	2019
Abstract	A GH49 dextranase gene DexKQ was cloned from marine bacteria Arthrobacter oxydans KQ11. It was recombinantly expressed using an Escherichia coli system. Recombinant DexKQ dextranase of 66 kDa exhibited the highest catalytic activity at pH 9.0 and 55 °C. kcat/Km of recombinant DexKQ at the optimum condition reached 3.03 s-1 μM-1, which was six times that of commercial dextranase (0.5 s-1 μM-1). DexKQ possessed a Km value of 67.99 µM against dextran T70 substrate with 70 kDa molecular weight. Thin-layer chromatography (TLC) analysis showed that main hydrolysis end products were isomalto-oligosaccharide (IMO) including isomaltotetraose, isomaltopantose, and isomaltohexaose. When compared with glucose, IMO could significantly improve growth of Bifidobacterium longum and Lactobacillus rhamnosus and inhibit growth of Escherichia coli and Staphylococcus aureus. This is the first report of dextranase from marine bacteria concerning recombinant expression and application in isomalto-oligosaccharide preparation.
File Format	PDF HTM / HTML
DOI	10.3390/md17080479
PubMed reference number	31430863
Journal	Medline
Volume Number	17
Alternate Webpage(s)	https://res.mdpi.com/d_attachment/marinedrugs/marinedrugs-17-00479/article_deploy/marinedrugs-17-00479.pdf
Alternate Webpage(s)	https://doi.org/10.3390/md17080479
Journal	Marine drugs
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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