NDLI: The CSB protein actively wraps DNA.

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The CSB protein actively wraps DNA.

Content Provider	Semantic Scholar
Author	Beerens, Nancy Kanaar, Roland Vermeulen, Wim
Copyright Year	2005
Abstract	The CSB protein is a member of the SWI2/SNF2 family of ATP-dependent chromatin remodeling factors and is essential for transcription-coupled DNA repair. The role of CSB in this DNA repair process is unclear, but the protein was found to remodel nucleosomes and alter DNA double helix conformation upon binding. Elucidating the nature of the change in DNA structure induced by CSB is of great interest for understanding the CSB mechanism of action. We analyzed the CSB.DNA complex by scanning force microscopy and measured a shortening of DNA contour length upon CSB binding in the presence of ATP. This DNA length reduction most likely results from DNA wrapping around the protein. Shorter DNA molecules were observed more frequently in the presence of non-hydrolyzable ATP analogues. These results suggest that DNA wrapping depends on ATP binding, whereas ATP hydrolysis results in unwrapping. We also provide evidence suggesting that CSB binds DNA as a dimer. DNA wrapping and unwrapping allows CSB to actively alter the DNA double helix conformation, which could influence nucleosomes and other protein-DNA interactions.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jbc.org/content/280/6/4722.full.pdf
PubMed reference number	15548521v1
Volume Number	280
Issue Number	6
Journal	The Journal of biological chemistry
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Triphosphate CD4 stimulated ATP immune response:MCnc:Pt:Bld:Qn DNA Repair ERCC6 gene Microscopy, Atomic Force Nucleosomes SWI2/SNF2 chromatin remodeling
Content Type	Text
Resource Type	Article

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