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GAPDH: the missing link between glycolysis and mitochondrial oxidative phosphorylation?
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ramzan, Rabia Weber, Petra María Linne, Uwe Vogt, Sebastian Dr. |
| Copyright Year | 2013 |
| Abstract | The main function of glycolysis and oxidative phosphorylation is to produce cellular energy in the form of ATP. In the present paper we propose a link between both of these energy-regulatory processes in the form of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and CytOx (cytochrome c oxidase). GAPDH is the sixth enzyme of glycolysis, whereas CytOx is the fourth complex of the mitochondrial oxidative phosphorylation system. In MS analysis, GAPDH was found to be associated with a BN-PAGE (blue native PAGE)-isolated complex of CytOx from bovine heart tissue homogenates. Both GAPDH and CytOx are highly regulated under normal energy metabolic conditions, but both of these enzymes are highly deregulated in the presence of oxidative stress. The interaction of GAPDH with CytOx could be the point of interest as it has already been shown that GAPDH protein damage results in a marked decrease in cellular ATP levels. On the other hand, decreasing the ATP/ADP ratio may ultimately result in switching off the allosteric ATP inhibition of CytOx leading to increased ROS (reactive oxygen species), cytochrome c release and apoptosis. Moreover, we have previously reported that allosteric ATP inhibition of CytOx is responsible for keeping the membrane potential at low healthy values, thus avoiding the production of ROS and this allosteric ATP inhibition is switched on at a high ATP/ADP ratio. So, in the present paper, we propose a scheme that could prove to be a link between these two enzymes and their role in the prevalence of diseases. |
| Starting Page | s281 |
| Ending Page | s282 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemsoctrans.org/content/ppbiost/41/5/1294.full.pdf |
| PubMed reference number | 24059522v1 |
| Alternate Webpage(s) | https://doi.org/10.1042/BST20130067 |
| DOI | 10.1042/bst20130067 |
| Journal | Biochemical Society transactions |
| Volume Number | 41 |
| Issue Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenosine Diphosphate Adenosine Triphosphate Apoptosis Bos taurus CYTOCHROME C OXIDASE Deregulation GAPDH gene Glyceraldehyde 3-Phosphate Glycolysis Membrane Potentials Metabolic Process, Cellular Mitochondrial Inheritance Oxidative Phosphorylation Oxidative Stress Polyacrylamide Gel Electrophoresis Rats, Inbred BN Reactive Oxygen Species |
| Content Type | Text |
| Resource Type | Article |
