NDLI: Rôle de la protéine ScFRK1 dans le développement du sac embryonnaire et son impact sur le guidage des tubes polliniques

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Rôle de la protéine ScFRK1 dans le développement du sac embryonnaire et son impact sur le guidage des tubes polliniques

Content Provider	Semantic Scholar
Author	Lafleur, Edith
Copyright Year	2010
Abstract	The Solanum chacoense Fertilization-Related Kinase 1 (ScFRK1) is a member of plant MAPKKK that is specifically expressed in ovules. ScFRK1 mRNA levels accumulate predominantly in the egg apparatus cells of the embryo sac at mature stage and decrease rapidly following pollination. These results suggest both preand post-fertilization roles in ovule development. Although no expression could be detected in mature pollen, FRK1 mRNAs could be detected in pollen mother cells. Transgenic plants expressing sense or antisense ScFRK1 showed no abnormal phenotype in vegetative tissues but produced seedless fruits upon pollination. A microscope-based examination of developing female gametophytes revealed that its formation did not progress further than the functional megaspore stage in affected transgenic plants and, as the levels of ScFRK1 mRNA decreased, the percentage of normal embryo sacs declined. Surprisingly, even in severely affected plants producing no or very few embryo sacs, pollination led to the production of parthenocarpic fruits. Similarly, viable pollen production declined with decreasing levels of ScFRK1 and this could be linked to affected mitosis I. Since embryo sac integrity is a prerequisite for pollen tube guidance, we devised a semi-in vivo pollen tube growth system to assess the ability of the ScFRK1 mutant ovules to attract pollen tubes. As expected, guidance was severely affected, confirming the involvement of the egg apparatus cells as the source of attracting molecules. Attraction was also determined to be highly speciesspecific and developmentally-regulated with the acquisition of attraction competence on anthesis day.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://papyrus.bib.umontreal.ca/xmlui/bitstream/handle/1866/3617/Lafleur_Edith_2009_memoire.pdf;jsessionid=B92FB4423B7E6BC38E868F47A176D9CE?sequence=4
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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