NDLI: High-resolution genotyping elucidates the epidemiology of group A streptococcus outbreaks.

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High-resolution genotyping elucidates the epidemiology of group A streptococcus outbreaks.

Content Provider	Semantic Scholar
Author	Stanley, John Desai, Meeta Xerry, Jacqueline Tanna, Asha Efstratiou, Androulla George, Robert
Copyright Year	1996
Abstract	Streptococcus pyogenes strains were genotyped by a combination of molecular methods for high- resolution epidemiologic studies of disease outbreaks. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the emm gene is reported. Alone or in conjunction with other molecular techniques (16S ribotyping, pulsed-field gel electrophoresis, and detection of exotoxin genes), PCR-RFLP could differentiate outbreak-related strains from contemporaneous background strains of the same M serotype. Three outbreaks were studied: pharyngitis in a boarding school (serotype M5), cross-infection in a hospital burn unit (serotype M76), and severe invasive disease in two elderly care homes (serotype R28). It was possible, for example, to identify within serotype R28 a clone with particular potential for invasive disease. In all cases, the four molecular methods yielded complementary results that were hierarchically related. Strains could be assigned to the outbreak or the background in a precise, reproducible, and rapid manner.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jid/174/3/10.1093/infdis/174.3.500/2/174-3-500.pdf?Expires=1491687401&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=ZwhPJC3vrhufaZ01AmVYNbbm3CVr1IPXJt4W8T6~8nIVJPskqoctmscnxyV1WNdQ9HWqypnjNq80ZMe-dAvwV~Kf~1WWoWqDM065Z8~E4kFWpz-Aye3qv5-YynUa6iEClQ14bGncmSKfnPcDBDFIIwBI23Ch-fpqbFKnALG7IYbJZofxM7HiIZNFpYPSySI1kek91d8j~KdC6XgEO1yscy4dnm1fwDOARmTmByKCOI4WiGIOSMlGdyl03AfUpNE9GIqs062F23-PA2q0oIHFNnbEsF1S99tFsf08atLEsw-5W5W0RgUhYQGjkp~GcdDyYodqu~5tVSXb5ZqzMpb3iA__
PubMed reference number	8769606v1
Volume Number	174
Issue Number	3
Journal	The Journal of infectious diseases
Language	English
Access Restriction	Open
Subject Keyword	Clone Electrophoresis, Gel, Pulsed-Field Epidemiology Exotoxins Gel Electrophoresis (lab technique) Genotype determination Molecular Genetic Technique PersonNameUse - assigned Pharyngitis Polymerase Chain Reaction Ribotyping Streptococcus agalactiae Streptococcus pyogenes
Content Type	Text
Resource Type	Article

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