NDLI: Antimikrobielle og antioksidative egenskaper ved butare (A. Esculenta), og bruk av butare som absorbent ved modifisert atmosfære pakking (MAP) av atlantisk laks (Salmo salar L.)

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Antimikrobielle og antioksidative egenskaper ved butare (A. Esculenta), og bruk av butare som absorbent ved modifisert atmosfære pakking (MAP) av atlantisk laks (Salmo salar L.)

Content Provider	Semantic Scholar
Author	Skadal, Markus Lie
Copyright Year	2018
Abstract	Initial tests were made to investigate the microbiological flora that exists on raw and dried, as well as dried gamma-irradiated Alaria Esculenta. This was investigated by cultivating samples of these algae on eight different media; Iron agar (total colony count), Long and Hammer (total psychrofile flora), MRS (lactic acid bacteria), STAA (Brochothrix thermosphacta), Pseudomonas agar (Pseudomonas spp.), anaerobic sporeforming media, petrifilm for Enterobacteriaceae and petrifilm for mold and yeast. Samples were also stored in modified atmosphere (CO2: N2, 60:40) at 4 °C for up to 14 days. No significant growth of microorganisms was observed on the gamma-irradiated algae in this experiment. The samples grown on Iron agar showed the greatest growth and these samples showed significantly (α = 0.05) lower growth for sterilized algae relative to raw and unsterilized algae. The aim of this master thesis was to determine if A. Esculenta had the potential to be used as an absorbent when storing a salmon fillet product (Salmo salar L.). The effect of A. esculenta on growth of microorganisms on salmon was compared to the use of a conventional absorbent. This analysis was carried out by packing salmon with and without A. esculenta in a modified atmosphere (CO2: N2, 50:50). Also, samples were made inoculated with Aeromonas Salmonicida to find out if A. esculenta could have an antimicrobial effect on this bacteria. All four groups were stored for 21 days at 4 °C in modified atmosphere (CO2: N2, 50:50). Samples were analyzed on day 0 and every subsequent third day. Samples were analyzed for growth of colony forming units by cultivating on three different media; Iron agar (total colony count), Starch and Ampicillin agar (Aeromonas spp.) and MRS-media (lactic acid bacteria). The samples with A. esculenta as an absorbent showed a significantly lower total colony count than the conventional absorbent samples, when cultivated on Iron agar. In particular, a large difference between the samples with and without A. esculenta was found in the non-inoculated samples cultivated on Iron agar. For samples grown on Starch and Ampicillin agar, there was only growth on the inoculated samples. The inoculated samples with algae showed significantly lower growth than the inoculated samples without algae. For samples grown on MRS-media there was a greater growth for the samples without algae than for the samples with algae. Analyzes for total phenol and antioxidant capacity were also performed for raw and dried (sterile and nonsterile) A. esculenta. These analyzes were carried out by extracting chemical components from A. esculenta with methanol:water (60:40) as solvent. The extracts were analyzed for total phenolic content by comparison with a gallic acid stock solution and the antioxidant capacity was found by a DPPH-method. Samples of sterile dried A. esculenta showed the highest phenolic content and the best antioxidant capacity. Based on the results, it can be concluded that A. esculenta has an antimicrobial effect on the general flora of an Atlantic salmon fillet product. It can also be concluded that the dried sterilized algae is better suited as an absorbent compared to raw or dried unsterilized algae. It can also be concluded that extracts of dried sterilized A. esculenta will yield higher phenolic content than extracts of raw or died unsterilized A. esculenta. One can also conclude that extracts of sterilized and dried A. esculenta will have better ability to scavenge free radicals than extracts of raw or dried unsterilized A. esculenta.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://ntnuopen.ntnu.no/ntnu-xmlui/bitstream/handle/11250/2564439/18489_FULLTEXT.pdf?isAllowed=y&sequence=1
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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