Design and evaluation of poly ( lactic-co-glyclic acid ) / poly ( vinyl alcohol ) / nano-hydroxyapatite hydrogels for cartilage tissue engineering in vitro

Content Provider	Semantic Scholar
Author	Zeng, Min Li, Ming-Qing Lin, Shaoru Su, Weiping Hu, Yihe
Copyright Year	2016
Abstract	A major challenge in cartilage tissue engineering is the scaffold. Poly (vinyl alcohol) (PVA) hydrogel has great potential because of its structure and material properties. But it still has some deficiencies, such as its insufficient mechanical property and poor biocompatibility. Nano-hydroxyapatite (nano-HA), as a bone repair material, could improve the mechanical property of scaffolds, while poly (lactic-co-glyclic acid) (PLGA), a degradable polymer, could improve the biocompatibility. So far, their synergic effects on PVA hydrogels had not been studied yet. In this paper, through solvent extraction/evaporation technique and freeze-thaw cycling method, the PLGA/PVA/nano-HA composite hydrogels were prepared. It was found that the morphological characterization of the composites could be changed by altering the content of raw materials. When the concentration of PVA was on a scale from 5 wt% to 15 wt%, the moisture content of the composites was in the ranged 74-84%, average pore size 80-118 μm, porosity 57-64%. Then the human mesenchymal stem cells (MSCs) were seeded in these composites to assess the biocompatibility and practicability in vitro by comparison to PVA/nano-HA hydrogels. The results of cell culture test showed that human MSCs were able to attach, grow and proliferate well in the composites. In addition, the composites could promote the proliferation and differentiation toward chondrocytes in vitro.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.ijcem.com/files/ijcem0024586.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article