EVALUACIÓN DE AISLAMIENTOS DE Trichoderma spp. y Bacillus spp. PARA EL CONTROL DE Phytophthora capsici Leonian CAUSANTE DE LA MARCHITEZ DEL PIMENTÓN

Content Provider	Semantic Scholar
Author	Padrón, Santander Isabel, Georgette
Copyright Year	2013
Abstract	The pepper wilt caused by Phytophthora capsici Leonian, is a disease that can occur at any age and causes crop yield losses of up to 100 %. To evaluate the use of Trichoderma harzianum Rifai and Bacillus subtilis (Ehrenberg) Cohn to control this pathogen, in the Plant Disease Clinic at the Agronomy Faculty of the Universidad Central de Venezuela, two trials were conducted with complete design randomized, one in vitro with the use of agar plates containing carrot (AZ), in order to evaluate, using the percent inhibition of growth (PIC) and the percent inhibition of sporulation (PIE), the behavior against the pathogen of 21 isolates of Trichoderma spp. and 20 of Bacillus spp. from different localities of Aragua, Guárico and Carabobo. Also, under greenhouse conditions, we assessed the effect of antagonists (Trichoderma spp. and Bacillus spp.) to inhibit wilt caused by P. capsici in pepper plants, establishing twelve treatments with ten replicates each and using a completely randomized design. The 12 treatments were individual and combined applications of the antagonists in the nursery, transplant and weekly soil with the pathogen and without the pathogen. Wilt symptoms were evaluated and plant growth parameters (height, fresh weight, dry weight of aerial parts as well as the number of flowers, fresh weight, dry weight and root volume). The isolates Tr12 (T. harzianum) of Múcura II and B1 (B. subtilis) of Ruiz Pineda were selected to present the highest PIC values (76.80 and 67.22 %) and PIE (88.17 and 87.67%), respectively. Treatment with the combination of T. harzianum (10conidia/mL) + B. subtilis (10cfu/mL) in the three application times, achieved a reduction in the incidence of wilt to 70% and significant increases in vegetative variables plant height (50.50 cm), fresh weight (43.18 g) and dry (5.78 g) of the aerial parts, fresh weight (10.53 g), dry weight (2.33 g) and root volume (15.80 mL). These results show the feasibility of the combined use of these biological control agents as one of the strategies to apply within crop management practices.
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Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article