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Multimer recognition and secretion by the non-classical secretion pathway in Bacillus subtilis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhao, Liuqun Chen, Jingqi Sun, Jibin Zhang, Dawei |
| Copyright Year | 2017 |
| Abstract | Non-classical protein secretion in bacteria is a common phenomenon. However, the selection principle for non-classical secretion pathways remains unclear. Here, our experimental data, to our knowledge, are the first to show that folded multimeric proteins can be recognized and excreted by a non-classical secretion pathway in Bacillus subtilis. We explored the secretion pattern of a typical cytoplasmic protein D-psicose 3-epimerase from Ruminococcus sp. 5_1_39BFAA (RDPE), and showed that its non-classical secretion is not simply due to cell lysis. Analysis of truncation variants revealed that the C- and N-terminus, and two hydrophobic domains, are required for structural stability and non-classical secretion of RDPE. Alanine scanning mutagenesis of the hydrophobic segments of RDPE revealed that hydrophobic residues mediated the equilibrium between its folded and unfolded forms. Reporter mCherry and GFP fusions with RDPE regions show that its secretion requires an intact tetrameric protein complex. Using cross-linked tetramers, we show that folded tetrameric RDPE can be secreted as a single unit. Finally, we provide evidence that the non-classical secretion pathway has a strong preference for multimeric substrates, which accumulate at the poles and septum region. Altogether, these data show that a multimer recognition mechanism is likely applicable across the non-classical secretion pathway. |
| Starting Page | 3 |
| Ending Page | 1607 |
| Page Count | 1605 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 28276482 |
| Alternate Webpage(s) | https://doi.org/10.1038/srep44023 |
| DOI | 10.1038/srep44023 |
| Journal | Scientific reports |
| Volume Number | 7 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alanine B anthracis XXX Ql Cult Cell secretion Cell septum Equilibrium Green Fluorescent Proteins Protein Secretion Protein Truncation Abnormality Secretory Pathway pathologic cytolysis |
| Content Type | Text |
| Resource Type | Article |
