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Joining of simian virus 40 DNA molecules at endonuclease R Eco Ri sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Vries, F. A. De Collins, Carol Jones Jackson, Dean A. |
| Copyright Year | 1976 |
| Abstract | DNA molecules cut with endonuclease R Eco Ri can be joined at Eco Ri cleavage sites by incubation with polynucleotide ligase. In order to define the optimum conditions for this reaction, linear Simian Virus 40 DNA molecules (SV40(Lri)) produced by endonuclease R Eco Ri cleavage of SV40 form i DNA were joined using polynucleotide ligases specified by bacteriophage T4 and Escherichia coli. We have determined that the concentration of the substrate DNA molecules is the most important factor determining the distribution of covalently joined product molecules into a variety of circular and linear monomeric and oligomeric species. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://deepblue.lib.umich.edu/bitstream/handle/2027.42/21728/0000120.pdf;jsessionid=1BFE0BB839B813A38D9F7B416013D1EC?sequence=1 |
| PubMed reference number | 181070v1 |
| Volume Number | 435 |
| Issue Number | 3 |
| Journal | Biochimica et biophysica acta |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Agarose Gel Bacteriophage T4 Bacteriophages Electrophoresis, Agar Gel Gel Electrophoresis (lab technique) HIV Ligase Polynucleotide Ligases Polynucleotides Simian virus 40 endonuclease |
| Content Type | Text |
| Resource Type | Article |
