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Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Zi Zheng, Wei Hsu, Chin-Ying Stephen Huang, Zhiwei |
| Copyright Year | 2016 |
| Abstract | We report the development and implementation of a rapid polarization-resolved hyperspectral stimulated Raman scattering (SRS) microscopy technique for label-free biomolecular imaging of the tooth. The hyperspectral SRS imaging technique developed covers both fingerprint (800–1800 cm−1) and high-wavenumber (2800–3600 cm−1) regions for tooth Raman imaging without fluorescence background interference with an imaging speed of 106 faster than confocal Raman imaging. Significant differences of hyperspectral SRS spectra among different tooth locations (e.g., dentin, enamel, and dentin-enamel junction) are observed, revealing the biochemical distribution differences across the tooth. Further polarization-resolved SRS imaging shows different polarization dependences related to the molecular orientation differences of various tooth locations. This work demonstrates the potential of polarization-resolved hyperspectral SRS imaging technique developed i... |
| Starting Page | 033701 |
| Ending Page | 033701 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1063/1.4939923 |
| Alternate Webpage(s) | http://www.focusonmicroscopy.org/2016/PDF/16041_Zheng.pdf |
| Alternate Webpage(s) | https://doi.org/10.1063/1.4939923 |
| Volume Number | 108 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
