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Force microscopy imaging of individual protein molecules with sub-pico Newton force sensitivity.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Patil, Shivprasad Martínez, Nicolás Fernández Lozano, José A. Rodríguez Garcia, Ricardo |
| Copyright Year | 2007 |
| Abstract | The capability of atomic force microscopes (AFM) to generate atomic or nanoscale resolution images of surfaces has deeply transformed the study of materials. However, high resolution imaging of biological systems has proved more difficult than obtaining atomic resolution images of crystalline surfaces. In many cases, the forces exerted by the tip on the molecules (1-10 nN) either displace them laterally or break the noncovalent bonds that hold the biomolecules together. Here, we apply a force microscope concept based on the simultaneous excitation of the first two flexural modes of the cantilever. The coupling of the modes generated by the tip-molecule forces enables imaging under the application of forces ( approximately 35 pN) which are smaller than those needed to break noncovalent bonds. With this instrument we have resolved the intramolecular structure of antibodies in monomer and pentameric forms. Furthermore, the instrument has a force sensitivity of 0.2 pN which enables the identification of compositional changes along the protein fragments. |
| File Format | PDF HTM / HTML |
| DOI | 10.1002/jmr.848 |
| PubMed reference number | 17918769 |
| Journal | Medline |
| Volume Number | 20 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://www.icmm.csic.es/forcetool/wp-content/uploads/2015/06/JMR_2007.pdf |
| Alternate Webpage(s) | https://doi.org/10.1002/jmr.848 |
| Journal | Journal of molecular recognition : JMR |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
