NDLI: Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin

Please wait, while we are loading the content...

Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin

Content Provider	Semantic Scholar
Author	Alvarez, Rafael Herrera Natal, Fabio Luis Nogueira Ribela, Maria Teresa C. P. Almeida, Beatriz Elane De Oliveira, João Ezequiel De Bartolini, Paolo
Copyright Year	2016
Abstract	Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in comparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained by this qualitative and quantitative physical-chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain of prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (tR= 27.9 min), which were related to the in vivo bioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weight and in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changes in the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.
Starting Page	459
Ending Page	466
Page Count	8
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://synapse.koreamed.org/Synapse/Data/PDFData/0118JVS/jvs-17-459.pdf
PubMed reference number	27297410
Alternate Webpage(s)	https://doi.org/10.4142/jvs.2016.17.4.459
DOI	10.4142/jvs.2016.17.4.459
Journal	Journal of veterinary science
Volume Number	17
Language	English
Access Restriction	Open
Subject Keyword	BLOC1S3 gene Biological Assay Chorionic Gonadotropin Equine Gonadotropins Heparin, Low-Molecular-Weight High pressure liquid chromatography procedure Ovulation Prepuberty Receptors, LH Retinitis Pigmentosa ovarian neoplasm
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in