NDLI: Bruceine D induces apoptosis in human chronic myeloid leukemia K562 cells via mitochondrial pathway.

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Bruceine D induces apoptosis in human chronic myeloid leukemia K562 cells via mitochondrial pathway.

Content Provider	Semantic Scholar
Author	Zhang, Jian-Ye Lin, Min-Ting Tung, Ho-Yi Tang, Si-Li Zhang, Ya-Zhou Tang, Yina Zhao, Zhong-Zhen Chen, Hu-Biao
Copyright Year	2016
Abstract	Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. In this study, we investigated the effects of bruceine D, which have been isolated from Brucea javanica (L.) Merr. on human chronic myeloid leukemia K562 cells. MTT assay was used to evaluate cell growth inhibition. Flow cytometry was performed to analyze mitochondrial membrane potential (ΔΨm). Western blot was applied to detect expression of cytochrome c, caspases-9, -3, PARP and other proteins. Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. It led to loss of ΔΨm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. In addition, bruceine D inhibited the phosphorylation of AKT and ERK. It's indicative that the potent anticancer activity of bruceine D be related to MAPK and PI3K pathways.
File Format	PDF HTM / HTML
PubMed reference number	27186433
Journal	Medline
Volume Number	6
Issue Number	4
Alternate Webpage(s)	http://www.ajcr.us/files/ajcr0007387.pdf
Alternate Webpage(s)	https://repository.hkbu.edu.hk/cgi/viewcontent.cgi?article=7089&context=hkbu_staff_publication
Journal	American journal of cancer research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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